Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: The validation of recombinant adenovirus BMP9 and siBMP9. ( A ) The expression of BMP9 in normal bladder mucosa ( n = 126), superficial bladder cancer ( n = 68), and infiltrating bladder cancer ( n = 62) in the Lee Bladder database. p = 0.007; ( B ) The expression levels of BMP9 in T24 and BIU-87 cells were detected by western blot; ( C ) The BMP9 was up-regulated in BIU-87 cells after being transfected with AdBMP9 compared to the control group; ( D ) The BMP9 was down-regulated in T24 cells after being transfected with AdsiBMP9 compared to the control group. Data are shown as mean ± SD. ** p < 0.01.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Biomarker Discovery, Recombinant, Expressing, Western Blot, Transfection, Control

Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: BMP9 up-regulated the expression of lncRNA UCA1 in bladder cancer cells. ( A ) Five common lncRNA were screened in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( B ) The expression of lncRNA UCA1 were verified in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( C ) The expression of lncRNA UCA1 were tested in T24 cells after being transfected with AdsiBMP9 by RT-PCR; ( D ) The inhibitory effect of siUCA1 were analyzed by RT-PCR in BIU-87 cells after being co-transfected with AdBMP9 and siUCA1. Data are shown as mean ± SD. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, vs. control groups.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Activation of the NLRP3 Inflammasome Pathway by Uropathogenic Escherichia coli Is Virulence Factor-Dependent and Influences Colonization of Bladder Epithelial Cells

doi: 10.3389/fcimb.2018.00081

Figure Lengend Snippet: The importance of different virulence factors for IL-1β release and caspase-1 activity. The bladder epithelial cell line 5637 (A–D) and a spontaneously transformed bladder epithelial cell line HBLAK (E) were infected with CFT073, CFT073Δpap, CFT073ΔfimH, CFT073ΔhlyA, CFT073ΔhlyA/pGNH404 and CFT073 fim L-ON at MOI 10 for 3 (A,C) and 6 h (B,D,E) . IL-1β release (A,B,E) and caspase-1 activity (C,D) were measured. Caspase-1 results are presented as fold increase of mean fluorescence units (MFU) compared to unstimulated control cells. Hemolysin activity on blood agar was evaluated for CFT073, CFT073 fim L-ON, and CFT073ΔhlyA after overnight incubation (F) . Data are presented as mean ± SEM ( n = 3 independent experiments). Asterisks denote statistical significance compared to respective unstimulated control (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: The human bladder epithelial cell line HBLAK (CELLnTEC Advanced Cell Systems AG, Bern, Switzerland) has been isolated from a healthy bladder and been spontaneously transformed providing the convenience of long-term cell growth without senescence.

Techniques: Activity Assay, Transformation Assay, Infection, Fluorescence, Incubation

Journal: Oncology Letters

Article Title: Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells

doi: 10.3892/ol.2013.1768

Figure Lengend Snippet: AKR1C2 protein expression in HT1376-CisR cells was markedly increased in comparison with the parental cells. AKR1C2 small interfering RNA reduced expression by ~80% in HT1376-CisR cells. AKR1C2 and β-tubulin exhibit discrete bands of the same molecular weight (AKR1C2, 37 kDa; β-tubulin, 51 kDa). AKR1C2, aldo-keto reductase family 1 member C2; CisR, cisplatin-resistant.

Article Snippet: The human HT1376 bladder cancer cell line used in this study was purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Expressing, Comparison, Small Interfering RNA, Molecular Weight

Journal: Oncology Letters

Article Title: Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells

doi: 10.3892/ol.2013.1768

Figure Lengend Snippet: Effect of AKR1C2 expression on cisplatin IC 50 values in parental and HT1376-CisR cells. Cells were treated with various cisplatin concentrations for 72 h, and then quantified using a cell counter. Each assay was performed in triplicate. Cell survival in the absence of cisplatin was set as 100%. (A) Silencing AKR1C2 restored HT1376-CisR cell response to cisplatin. (B) Inhibition of AKR1C2 by 100 μM 5β-cholanic acid restored the HT1376-CisR response to cisplatin. * P<0.05, vs. HT1376-CisR. Bars indicate standard deviation. AKR1C2, aldo-keto reductase family 1 member C2; CisR, cisplatin-resistant.

Article Snippet: The human HT1376 bladder cancer cell line used in this study was purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Expressing, Inhibition, Standard Deviation

Journal: Oncology Letters

Article Title: Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells

doi: 10.3892/ol.2013.1768

Figure Lengend Snippet: Effect of cisplatin on intracellular ROS in HT1376 cells. Exposure to cisplatin increased the levels of intracellular ROS in HT1376 cells in a dose-dependent manner. * P<0.05, vs. HT1376 cells cultured without cisplatin. Bars indicate standard deviation. ROS, reactive oxygen species.

Article Snippet: The human HT1376 bladder cancer cell line used in this study was purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Cell Culture, Standard Deviation

Journal: Oncology Letters

Article Title: Cisplatin resistance by induction of aldo-keto reductase family 1 member C2 in human bladder cancer cells

doi: 10.3892/ol.2013.1768

Figure Lengend Snippet: Relative values of intracellular ROS measured using a 2,7-dichlorodihydrofluorescein diacetate probe. (A) Basal intracellular ROS levels in HT1376, HT1376-CisR and HT1376-CisR cells transiently transfected with AKR1C2 small interfering RNA [HT1376-CisR-AKR1C2(−)]. * P<0.05 and $ P<0.05, vs. HT1376 and HT1376-CisR cells cultured without cisplatin, respectively. (B) Effect of 10 −4 M cisplatin exposure on intracellular ROS in these cells. (C) Effect of 5 μM menadione on intracellular ROS in these cells. * P<0.05 vs. control cells cultured without cisplatin or menadione. Bars indicate standard deviation. AKR1C2, aldo-keto reductase family 1 member C2; CisR, cisplatin-resistant; ROS, reactive oxygen species.

Article Snippet: The human HT1376 bladder cancer cell line used in this study was purchased from DS Pharma Biomedical (Osaka, Japan).

Techniques: Transfection, Small Interfering RNA, Cell Culture, Control, Standard Deviation

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

doi: 10.31557/APJCP.2024.25.3.921

Figure Lengend Snippet: Cytotoxic effect of BrD in T24 Cells Treated for 72 h White Arrow Indicates Morphological Changes in Treated Cells. BrD = Brucein D, Dox = Doxorubicin, Doc = Docetaxel

Article Snippet: Cell Line T24 Bladder Cancer The bladder cancer cell line T24 was purchased from the ECΑCC (European Collection of Authenticated Cell Cultures) with cat-pack number 85061107-1 VL.

Techniques:

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

doi: 10.31557/APJCP.2024.25.3.921

Figure Lengend Snippet: Dose-Response of BrD Treatment (0.01 – 100 µg/mL) in T24 cells for 72 h

Article Snippet: Cell Line T24 Bladder Cancer The bladder cancer cell line T24 was purchased from the ECΑCC (European Collection of Authenticated Cell Cultures) with cat-pack number 85061107-1 VL.

Techniques:

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

doi: 10.31557/APJCP.2024.25.3.921

Figure Lengend Snippet: Viability assay by Staining with (A) Calceіn AM (green) and PI (red) in T24 Cells Treated with a Graded Dose of BrD, Doxorubicin, and Docetaxel for 72 h. The percentage of cells stained with PI represents dead cells (B) The percentage of dead cells showed an increase in a dose-dependent manner (ns indicates non-significant, * significant difference p< 0.05, ** p<0.01, *** p<0.001, **** p<0.0001)

Article Snippet: Cell Line T24 Bladder Cancer The bladder cancer cell line T24 was purchased from the ECΑCC (European Collection of Authenticated Cell Cultures) with cat-pack number 85061107-1 VL.

Techniques: Viability Assay, Staining

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Cytotoxicity and Apoptosis Studies of Brucein D against T24 Bladder Cancer Cells

doi: 10.31557/APJCP.2024.25.3.921

Figure Lengend Snippet: Apoptosis-Inducing Effects of T-24 Cells: (A) Hoeϲhѕt33342 staining shows condensation of chromatin in apoptotic cells; arrowheads = apoptotic nuclei. (B) CTCF values of T-24 cells treated with BrD, doxorubicin, and docetaxel at IC50 concentrations, shows an increase in cell fluorescence that indicates an increase of chromatin condensation in all treatment group compared to control (C) Measurement of the apoptotic nuclei percentage in T24 cells treated with BrD, doxorubicin, and docetaxel in IC50 concentrations shows an increase of apoptotic nuclei percentage in all treatment group compared to control. (D) Fragmentation of T24 genomic DNA treated with BrD, doxorubicin, and docetaxel at IC50 concentration; a 1 kb DNA ladder (250–10,000 bp Τhermo Fіsher) was used as a basic measurement; M: 1 kb marker DNA ladder; C: control. Arrows indicate genomic DNA and fragmented DNA. (ns indicates non-significant, * significant difference p< 0.05, ** p<0.01, *** p<0.001, **** p<0.0001)

Article Snippet: Cell Line T24 Bladder Cancer The bladder cancer cell line T24 was purchased from the ECΑCC (European Collection of Authenticated Cell Cultures) with cat-pack number 85061107-1 VL.

Techniques: Staining, Fluorescence, Concentration Assay, Marker